CD68 (DKFZp686M18236, GP110, Macrophage Antigen CD68 (Microsialin), Macrosialin, Scavenger Receptor Class D Member 1, SCARD1) (MaxLight 490)

Mab

MaxLight 490

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.

Rat spleen cells

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73, 000.

CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by Flow Cytometry (Not Tested) using cell permeablization. CD68 is a 110kD transmembrane (Type I) glycoprotein consisting of a polypeptide of 37408D (354aa) predicted from the cDNA sequence that is highly expressed by human monocytes and tissue macrophages. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes, lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.

The antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat. It is expressed predominantly on the lysosomal membrane and lightly on the cell surface.

Applications:
Suitable for use in Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: Membrane permeabilization is required.
Immunohistochemistry (Paraffin): Requires protein digestion pre-treatment of paraffin sections (e.g. trypsin or pronase)
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0- Ag14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.