ERK1 (Extracellular Signal-regulated Kinase 1,ERK-1,Esrk1,ERT2,Insulin-stimulated MAP2 Kinase,MAP Kinase Isoform p44,Mitogen-activated Protein Kinase 3,MAP Kinase 3,MAPK 3,Mapk3,Mitogen-activated Protein Kinase 1,MAP Kin

Mab

MaxLight 650

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.

Synthetic Peptide corresponding to a section of the C-terminus of rat ERK1

MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250, 000.

Mitogen Activated Protein Kinases (MAPKs) play pivotal roles in mediating signal transduction from the cell surface to the nucleus. These kinases are encoded by distinct genes and together form a family of kinases whose activation is dependent upon dual phosphorylation on specific threonine and tyrosine residues. In yeast, a number of different MAP Kinases have been identified and are activated by distinct signaling pathways. In mammalian cells, the best characterized sub-group of the MAP Kinase family are the Extracellular Signal Regulated Kinases (ERKs). To date, at least 4 distinct ERKs have been identified including: ERK1 (p44/p43), ERK2 (p42/p43), ERK3 (p62), and ERK4 (p45). Analysis of cDNAs encoding MAP Kinase suggest that numerous other ERKs may exist. MAP Kinase has been shown to phosphorylate numerous proteins including: RSK (4) , c-Fos, c-Jun, c-Myc, c-raf, MAP2, and MEK (5) . MAP Kinase is directly activated when phosphorylated by MEK and indirectly stimulated by many factors (6, 7, 8) .

Applications:
Suitable for use in FLISA, Western Blotting, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
FLISA: 0.1–1ug/ml
Immunoprecipitation (11) : 2–5ug
Western Blotting (9, 10) : 1ug/ml

Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.