MeCP2 (Methyl CpG Binding Protein 2 (Rett Syndrome), AUTSX3, MRX16, MRX79, PPMX, RTS, RTT) (HRP)

Antibodies / Antibodies-Disease Markers

-20°C

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

KLH-conjugated, synthetic peptide corresponding to aa465-478 of mouse MeCP2 (C-PRPNREEPVDSRTP) with an N-terminal cysteine added for conjugation purposes

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Immunoblot Analysis: 0.5-2ug/ml of this lot detected MeCP2 in mouse heart nuclear extracts and a previous lot detected MeCP2 in mouse brain nuclear extracts and L6 nuclear extracts.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.