Neurofilament M, 160kD (NF-M) (HRP)

Antibodies / Antibodies-Neurofilament

-20°C

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).

Rat neurofilaments

Neurofilament proteins (NFPs) are a macromolecular complex comprised of 3 polypeptides designated as NF-L, NF-M and NF-H. NFPs are found in the perikarya, particularly in neuronal axons throughout the central and peripheral nervous system. Since NFPs are major structural proteins and biochemically quite stable, antibodies to NFPs are useful probes in studies of neuronal expression, morphology, connectivity and pathology. The presence or absence of NFP in a variety of nervous system or neuroendocrine tumors can provide useful information about the original cell type of the tumor. In addition, the normal NFP staining pattern is altered in a variety of human diseases including Alzheimer’s disease, diffuse cortical Lewy body disease, Parkinson’s disease and amyotrophic lateral sclerosis (Lou Gehrig’ disease). Alterations in the NFP expression pattern are also seen in most toxin-induced, sporadic and heriditary axonopathies occurring in humans and animals.

Applications:
Suitable for use in Flow Cytometry (Not Tested), ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry (Not Tested):
ELISA: 0.1–0.5ug/ml
Immunohistochemistry: 5–10ug/ml (paraffin-embedded or frozen sections)
Immunoprecipitation: 2–5ug
Immunoblotting(1): 0.5–1.0ug/ml

Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.