SRF (Serum Response Factor, c-fos Serum Response Element-binding Transcription Factor, MADS Family Member) (HRP)

Antibodies / Antibodies-Transcription Factors

-20°C

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

Synthetic human SRF peptide, aa406-508. Cellular Localization: Nucleus.

Serum response factor (SRF) is a nuclear transcription factor that binds to the serum response element (SRE), and is essential for embryonic development of the mesoderm, as well as for the maintenance of adult muscle cells and neurons. As this protein is involved in the downstream regulation of many immediate-early genes, it participates in a variety of cell functions, including cell cycle, apoptosis, cell growth, and cell differentiation. SRF is most commonly activated by serum, LPA, LPS, TPA, growth factors, including TNFalpha, as well as antioxidants and UV light.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Immunocytochemistry: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.