Tubulin,beta (Beta Tubulin,Tubulin beta Polypeptide,Tubulin beta-2 Chain,DAQB-47P19.9-001,dJ40E16.7,M40,MGC117247,MGC16435,OK/SW-cl.56,TUBB,TUBB Protein,TUBB 1,TUBB1,TUBB 2,TUBB2,TUBB 5,TUBB5,XXbac-BCX111D4.2-001) (MaxLi

Mab

MaxLight 490

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.

b-tubulin from sea urchin (S. purpuratus) sperm.

MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73, 000.

The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. The effects on tubulin messenger RNA levels and tubulin protein synthesis when treating cells with microtubule-depolymerizing drugs or when directly microinjecting cells with tubulin suggest that non-polymerized tubulin depresses its own synthesis. Accumulation of tubulin protein and an increased array of microtubules have been associated with contractile dysfunction in cardiac myocytes after pressure overload in vivo. Studies have also shown that cardiac activity can increase the amount of beta-tubulin in rat cardiac myocytes. Tubulin production in cultured cardiac myocytes can be regulated directly by mechanical forces. In mechanically challenged hearts, the accumulation of beta-tubulin and the development of contractile dysfunction may be directly related to the mechanical forces imposed on the myocardium during the onset and progression of cardiovascular disease.

Applications:
Suitable for use in Western Blot and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Western Blot (Human, mouse and rat): 1-3ug/ml
Immunofluorescence (C. elegans): 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse NIH3T3 fibroblast cells, rat brain, and mouse testis.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.