VEGFR-2 (Vascular Endothelial Growth Factor Receptor 2, VEGFR-2, FLK-1, KDR) (VEGFR2, VEGFR 2, Protein-tyrosine kinase receptor flk-1, Fetal liver kinase 1, Kinase NYK, FLK1, KDR, Kinase insert domain receptor, KRD1, Ly73, Protein tyrosine kinas

Pab

MaxLight 550

4°C Do Not Freeze

Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550.

Synthetic peptide derived from the N-terminal region of the mouse vascular endothelial growth factor receptor-2 (VEGFR-2, FLK-1, KDR) protein.

Vascular endothelial growth factor receptor-2 (VEGFR-2), also known as fetal liver kinase 1 (FLK-1) in mouse and KDR in human, is a transmembrane tyrosine kinase that plays an important role in the development of the vascular system and the vascularization of a wide variety of tumors. Binding of VEGF to VEGFR-2 leads to tyrosine phosphorylation of the dimerized receptor and subsequent phosphorylation of SH2-containing intracellular signaling proteins, including phospholipase C, Src family tyrosine kinase and phosphatidylinositol 3-kinase (PI3K) adaptor molecules, SHC, NCK, and Ras GTPase-activating protein. VEGFR-2 is expressed in cultured human umbilical vein endothelial cells (HUVEC), Hela cells, WM 35 melanoma cells, WM 9 melanoma cells, as well as mouse NIH3T3, and Balb/c 3T3 cells.
In a single study of 115 paraffin-embedded cancer specimens analyzed by immunohistochemistry, VEGFR-2 expression was observed in bladder tumors, breast cancer, intestinal cancer and lung cancer, suggesting a direct role for VEGF as an autocrine growth factor in a variety of human cancers.

Applications:
Suitable for use in FLISA and Western Blot. Other applications not tested.

Recommended Dilution:
FLISA: 0.1-1ug/ml
Western Blot: 1–5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.