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MET/HGFR Antibody

MET/HGFR Antibody

Hersteller	Abcepta
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Specific against	other
Isotype	IgG1
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applikationen	WB, IF
Menge	400 ul
Host	Mouse
ArtNr	ABC-AM1005a
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Primary Accession	P08581
Application	WB, IF
Bio Background	The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
Bio References	References for protein: 1.MET receptor sequence variants R970C and T992I lack transforming capacity. Tyner JW, et al. Cancer Res, 2010 Aug 1. PMID 20670955. 2.Further evidence for the role of MET in autism susceptibility. Thanseem I, et al. Neurosci Res, 2010 Oct. PMID 20615438. 3.Increased HGF and c-Met in muscle tissues of polymyositis and dermatomyositis patients: beneficial roles of HGF in muscle regeneration. Sugiura T, et al. Clin Immunol, 2010 Sep. PMID 20580899. 4.Correlation between hepatocyte growth factor receptor and vascular endothelial growth factor-A in breast carcinoma. Gisterek I, et al. Folia Histochem Cytobiol, 2010 Jan 1. PMID 20529820. 5.MET overexpressing chordomas frequently exhibit polysomy of chromosome 7 but no MET activation through sarcoma-specific gene fusions. Grabellus F, et al. Tumour Biol, 2010 Jun. PMID 20512480. References for HepG2 cell line: 1. Knowles BB, et al. (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499.[ PubMed: 6248960]. 2. Darlington GJ, et al. (1987). Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354.[PubMed: 3034851]. 3. Ihrke, G; Neufeld, EB; Meads, T; Shanks, MR; Cassio, D; Laurent, M; Schroer, TA; Pagano, RE et al. (1993). "WIF-B cells: an in vitro model for studies of hepatocyte polarity". Journal of Cell Biology 123 (6): 1761–1775. [PubMed:7506266]. 4. Mersch-Sundermann, V.; Knasmüller, S.; Wu, X. J.; Darroudi, F.; Kassie, F. (2004). "Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents". Toxicology 198 (1–3): 329–340. [PubMed:15138059].
Clonality	Monoclonal
Clone/Animal Names	4AT247
Gene ID	4233
Gene Name	MET
Subtitle	Purified Mouse Monoclonal Antibody (Mab)
Reactivity	H, M
Legend image 1	Fluorescent confocal image of HepG2 cells stained with MET/HGFR antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1005a MET/HGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the MET immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000105976).
Type image 1	IF
Dilution image 1	1:100
Legend image 2	Western blot analysis of lysates from HepG2, H. liver, HT-29 cell line (from left to right), using MET/HGFR Antibody(4AT247. 86. 63). 4AT247. 86. 63 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Detection of endogenous Met in HepG2 cell line. 10 μg/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Abgent's different anti-Met monoclonal antibodies that are Cat# AM1001-1005. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab (Cat# AM1000).
Type image 3	WB
Dilution image 3	1:500~1000
Isotype	Mouse IgG1
Calculated MW	155541
Antigen Source	HUMAN
Target/Specificity	This monoclonal antibody is generated from mice immunized with purified recombinant protein encoding the catalytic domain of human Met.
NCBI Accession	NP_000236.2; NP_001120972.1

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ABC-AM1005a-datasheet.pdf

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Menge: 400 ul

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