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AFP Antibody

AFP Antibody

Hersteller	Abcepta
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Specific against	other
Isotype	IgG2b kappa
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applikationen	WB, IHC-P
Menge	200 ul
Host	Mouse
ArtNr	ABC-AM8611b
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Primary Accession	P02771
Antigen Type	Recombinant Protein
Application	WB, IHC-P
Bio Background	Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Bio References	Morinaga T., et al.Proc. Natl. Acad. Sci. U.S.A. 80:4604-4608(1983). Gibbs P.E.M., et al.Biochemistry 26:1332-1343(1987). Ota T., et al.Nat. Genet. 36:40-45(2004). Sakai M., et al.J. Biol. Chem. 260:5055-5060(1985). McVey J.H., et al.Hum. Mol. Genet. 2:379-384(1993).
Clonality	monoclonal
Clone/Animal Names	1087CT8.3.5
Gene ID	174
Gene Name	AFP
Subtitle	Purified Mouse Monoclonal Antibody (Mab)
Reactivity	H
Legend image 1	Anti-AFP Antibody at 1:500 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:500
Legend image 2	Anti-AFP Antibody at 1:2000 dilution + human fetal Liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	AM8611b staining AFP in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 3	IHC-P
Dilution image 3	1:25
Isotype	IgG2b, k
Calculated MW	68678
Antigen Source	HUMAN
Target/Specificity	This AFP antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 281-609 amino acids from human AFP.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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ABC-AM8611b-datasheet.pdf

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Menge: 200 ul

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Lieferung vsl. bis 04.07.2024

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