CRK Antibody

Recombinant Protein

1. Seikagaku. 2009 May; 81(5):361-76.  2. Mol Cancer Res. 2009 Sep; 7(9):1582-92.

1398

Purified Mouse Monoclonal Antibody

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);

1/10000

WB

Figure 2: Western blot analysis using CRK mAb against HEK293 (1) and CRK(AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate.

1/500 - 1/2000

IHC

Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CRK mouse mAb with DAB staining.

1/200 - 1/1000

IF

Figure 6: Flow cytometric analysis of MCF-7 cells using CRK mouse mAb (blue) and negative control (red).

1/200 - 1/400

33831

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

Purified recombinant fragment of human CRK expressed in E. Coli.