VEGFA Antibody

P15692

1.Oncol Rep. 2014 Dec; 32(6):2359-64. 2.Eur J Cancer. 2014 Nov; 50(16):2855-65.

7422

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng); Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

1/10000

WB

Figure 3:Western blot analysis using VEGFA mAb against HEK293 (1) and VEGFA (AA: 207-371)-hIgGFc transfected HEK293 (2) cell lysate.

1/500 - 1/2000

WB

Figure 5:Immunofluorescence analysis of GC-7901 cells using VEGFA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

1/200 - 1/1000

IF

Figure 7:Immunofluorescence analysis of HepG2 cells using VEGFA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

1/200 - 1/1000

27042

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.

Purified recombinant fragment of human VEGFA (AA: 207-371) expressed in E. Coli.