Hersteller |
Abcepta
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
other |
Isotype |
Ig |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applikationen |
WB, IF, IHC-P |
Menge |
400 ul |
Host |
Rabbit |
ArtNr |
ABC-AP1214a |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Primary Accession |
Q9GZV8 |
Antigen Type |
Synthetic Peptide |
Application |
IF, IHC-P, WB |
Bio Background |
Similar to acetylation and phosphorylation, histone methylation at the N terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET domain containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET domain family, and another 17 make up the PR domain family that is related to the SET domain family.PRDM14 is part of a family of PR domain genes that are involved in tumorigenesis. It may function as a transcription factor. |
Bio References |
Xiao, B., et al., Curr. Opin. Struct. Biol. 13(6):699-705 (2003).Dias Neto, E., et al., Proc. Natl. Acad. Sci. U.S.A. 97(7):3491-3496 (2000).Jiang, G.L., et al., Histol. Histopathol. 15(1):109-117 (2000). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB1405 |
Gene ID |
63978 |
Gene Name |
PRDM14 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
IFanalysis of PRDM14 in breast cancer cells. SKBr-3 cells were transfected with pCMV-Tag2A or pCMV-PRDM14 and then labeled with anti-PRDM14 antibody. The nucleus was stained with DAPI. |
Type image 1 |
IF |
Dilution image 1 |
1:10~50 |
Legend image 2 |
Western blot analysis of PRDM14 polyclonal antibody (Cat. #AP1214a) in A549 cell lysate. PRDM14 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Type image 3 |
IHC-P |
Dilution image 3 |
1:50~100 |
Isotype |
Rabbit Ig |
Calculated MW |
64062 |
Antigen Region |
1-30 |
Antigen Source |
HUMAN |
Target/Specificity |
This PRDM14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PRDM14. |
NCBI Accession |
NP_078780.1 |
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