CDKN1A Antibody (C-term)

Synthetic Peptide

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.

Polyclonal

1026

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Fluorescent image of A549 cell stained with CDKN1A Antibody (C-term)(Cat#AP13468b).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CDKN1A primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).CDKN1A immunoreactivity is localized to Nucleus significantly.

1:10~50

WB

CDKN1A Antibody (C-term) (Cat. #AP13468b) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CDKN1A antibody detected the CDKN1A protein (arrow).

1:1000

IHC-P

NP_000380.1; NP_510867.1

18119

HUMAN

NP_000380.1; NP_001207706.1; NP_001207707.1; NP_510867.1