Hersteller |
Abcepta
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
other |
Isotype |
Ig |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applikationen |
WB |
Menge |
400 ul |
Host |
Rabbit |
ArtNr |
ABC-AP21032a |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Primary Accession |
P52927 |
Application |
WB |
Bio Background |
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. |
Bio References |
Manfioletti G., et al.Nucleic Acids Res. 19:6793-6797(1991).Manfioletti G., et al.Gene 167:249-253(1995).Zhou X., et al.Nucleic Acids Res. 24:4071-4077(1996).Carninci P., et al.Science 309:1559-1563(2005).Di Agostino S., et al.Mol. Biol. Cell 15:1224-1232(2004). |
Clonality |
Polyclonal |
Clone/Animal Names |
RB51072 |
Gene ID |
15364 |
Gene Name |
Hmga2 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M |
Legend image 1 |
Western blot analysis of lysates from HepG2, mouse NIH/3T3 cell line (from left to right), using Mouse Hmga2 Antibody (N-term)(Cat. #AP21032a). AP21032a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Western blot analysis of lysate from NCCIT cell line, using Mouse Hmga2 Antibody (N-term)(Cat. #AP21032a). AP21032a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug. |
Type image 2 |
WB |
Dilution image 2 |
1:1000 |
Legend image 3 |
All lanes : Anti-Hmga2 Antibody (N-term) at 1:2000 dilutionLane 1: HepG2 whole cell lysatesLane 2: 293T whole cell lysatesLane 3: MDA-MB-231 whole cell lysatesLane 4: NIH/3T3 whole cell lysatesLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 12 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 3 |
WB |
Dilution image 3 |
1:2000 |
Other Accession |
P52926 |
Isotype |
Rabbit Ig |
Calculated MW |
11819 |
Antigen Source |
HUMAN |
Target/Specificity |
This Mouse Hmga2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 26-59 amino acids from the N-terminal region of Mouse Hmga2. |
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