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PRG4 Antibody (N-Term)

PRG4 Antibody (N-Term)

Hersteller	Abcepta
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Specific against	other
Isotype	Ig
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IF
Menge	200 ul
Host	Rabbit
ArtNr	ABC-AP22357a
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Primary Accession	Q92954
Antigen Type	Synthesized Peptide
Application	WB, IF
Bio Background	Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.
Bio References	Turner K.J., et al.Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases. Ota T., et al.Nat. Genet. 36:40-45(2004). Gregory S.G., et al.Nature 441:315-321(2006). Jones A.R., et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Flannery C.R., et al.Biochem. Biophys. Res. Commun. 254:535-541(1999).
Clonality	polyclonal
Clone/Animal Names	RB57887
Gene ID	10216
Gene Name	PRG4
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (human cervical epithelial adenocarcinoma cell line) cells labeling PRG4 with AP22357a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
Type image 1	IF
Dilution image 1	1:25
Legend image 2	Anti-PRG4 Antibody (N-Term) at 1:500 dilution + Human liver whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 151 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:500
Predicted	H
Isotype	Rabbit Ig
Calculated MW	151061
Antigen Source	HUMAN
Target/Specificity	This PRG4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 176-210 amino acids from the human region of human PRG4.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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ABC-AP22357a-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 200 ul

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Lieferung vsl. bis 04.07.2024

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