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AGL Antibody (C-term)

AGL Antibody (C-term)

Hersteller	Abcepta
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Specific against	other
Isotype	Ig
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, IF
Menge	400 ul
Host	Rabbit
ArtNr	ABC-AP2402B
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Primary Accession	P35573
Antigen Type	Synthetic Peptide
Application	WB, IF
Bio Background	AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1, 6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.
Bio References	Horinishi, A., et al., J. Hum. Genet. 47(2):55-59 (2002). Bao, Y., et al., Genomics 38(2):155-165 (1996). Yang, B.Z., et al., J. Biol. Chem. 267(13):9294-9299 (1992). Yang-Feng, T.L., et al., Genomics 13(4):931-934 (1992). Bao, Y., et al., Gene 197 (1-2), 389-398 (1997).
Clonality	Polyclonal
Clone/Animal Names	RB4978
Gene ID	178
Gene Name	AGL
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Expression of myc-GS causes wild type but not the ÃCBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL ÃCBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Confocal immunofluorescent analysis of AGL Antibody (C-term)(Cat#AP2402b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Type image 2	IF
Dilution image 2	1:10~50
Legend image 3	Western blot using anti-AGL (C-term) antibody (AP2402c) at 1:1000 dilution. A total of 20 ug of lysates was loaded for each tissue. Data courtesy of Dr. Alan Cheng, Department of Internal Medicine, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Anti-AGL Antibody (C-term) at 1:8000 dilution + human skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 175 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 4	WB
Dilution image 4	1:8000
Isotype	Rabbit Ig
Calculated MW	174764
Antigen Region	1479-1510
Antigen Source	HUMAN
Target/Specificity	This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1479-1510 amino acids from the C-terminal region of human AGL.
NCBI Accession	NP_000019.2; NP_000633.2; NP_000634.2; NP_000635.2; NP_000636.2; NP_000637.2

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ABC-AP2402B-datasheet.pdf

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Menge: 400 ul

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Lieferung vsl. bis 04.07.2024

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