Bio Background |
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. MGAT1 (UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1, 2-N-acetylglucosaminyltransferase I) is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. |
Bio References |
Tan, J., et al., Eur. J. Biochem. 231(2):317-328 (1995).Kumar, R., et al., Glycobiology 2(4):383-393 (1992).Hull, E., et al., Biochem. Biophys. Res. Commun. 176(2):608-615 (1991).Kumar, R., et al., Proc. Natl. Acad. Sci. U.S.A. 87(24):9948-9952 (1990).Yip, B., et al., Biochem. J. 321 (Pt 2), 465-474 (1997). |
Legend image 2 |
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |