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MMP3 Antibody (Center)

MMP3 Antibody (Center)

ArtNr	ABC-AP6211a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	MMP-3, MMP3, STMY1, Stromelysin-1, Matrix metalloproteinase-3, Transin-1, SL-1
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Primary Accession	P08254
Antigen Type	Synthetic Peptide
Application	FC, IHC-P, WB
Bio Background	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP3 is an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation.
Bio References	Sage, E.H., et al., J. Biol. Chem. 278(39):37849-37857 (2003). Matsuyama, A., et al., Circulation 108(12):1469-1473 (2003). Mercapide, J., et al., Int. J. Cancer 106(5):676-682 (2003). Bodemer, C., et al., J. Invest. Dermatol. 121(2):273-279 (2003). Kang, M.K., et al., Exp. Cell Res. 287(2):272-281 (2003).
Clonality	Polyclonal
Clone/Animal Names	RB02015-02016
Gene ID	4314
Gene Name	MMP3
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	All lanes : Anti-MMP3 Antibody (H313) at 1:1000 dilution Lane 1: U118MG whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 2	IHC-P
Dilution image 2	1:10~50
Legend image 3	Flow cytometric analysis of HepG2 cells using MMP3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3	FC
Dilution image 3	1:10~50
Other Accession	NP_002413
Isotype	Rabbit Ig
Calculated MW	53977
Antigen Region	298-327
Antigen Source	HUMAN
Target/Specificity	This MMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-327 amino acids from the Central region of human MMP3.
NCBI Accession	NP_002413.1

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