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ABCG1 Antibody (N-term)

ArtNr ABC-AP6529A
Hersteller Abcepta
Menge 400 ul
Kategorie
Typ Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen WB, FC, IF, IHC-P
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products ABCG1, ABC8, ATP-binding cassette sub-family G member 1, ATP-binding cassette transporter 8, White protein homolog, WHT1
Lieferbar
Primary Accession
P45844
Antigen Type
Synthetic Peptide
Application
WB, IHC-P, IF, FC
Bio Background
ABCG1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types.
Bio References
Furuyama, S., J. Atheroscler. Thromb. 16 (3), 194-200 (2009)
Stefulj, J., Circ. Res. 104 (5), 600-608 (2009)
Mauerer, R., Exp. Mol. Med. 41 (2), 126-132 (2009)
Clonality
Polyclonal
Clone/Animal Names
RB19678
Gene ID
9619
Gene Name
ABCG1 (HGNC:73)
Subtitle
Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H, M
Legend image 1
Confocal immunofluorescent analysis of ABCG1 Antibody (N-term)(Cat#AP6529a) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Type image 1
IF
Dilution image 1
1:10~50
Legend image 2
All lanes : Anti-ABCG1 Antibody (N-term) at 1:2000 dilution
Lane 1: Human liver lysate
Lane 2: Mouse liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 76 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2
WB
Dilution image 2
1:2000
Legend image 3
ABCG1 Antibody (N-term) (Cat. #AP6529a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ABCG1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 3
IHC-P
Dilution image 3
1:50~100
Legend image 4
ABCG1 Antibody (N-term) (Cat.#AP6529a) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4
FC
Dilution image 4
1:10~50
Other Accession
Q64343
Isotype
Rabbit Ig
Calculated MW
75592
Antigen Region
168-195
Antigen Source
HUMAN
Target/Specificity
This ABCG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-195 amino acids from the N-terminal region of human ABCG1.
NCBI Accession
NP_004906.3; NP_058198.2; NP_997057.1; NP_997510.1; NP_997511.1; NP_997512.1

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 400 ul
Lieferbar: In stock
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