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KRT14 Antibody (Center)

KRT14 Antibody (Center)

ArtNr	ABC-AP6713c
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	KRT14, Keratin, K14, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14
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Primary Accession	P02533
Antigen Type	Synthetic Peptide
Application	FC, IHC-P, WB
Bio Background	KRT14 is a member of the keratin family, the most diverse group of intermediate filaments. This protein, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells.
Bio References	Barcelos, A.C., J. Cutan. Pathol. 36 (6), 647-654 (2009) Romano, R.A., PLoS ONE 4 (5), E5623 (2009)
Clonality	Polyclonal
Clone/Animal Names	RB18415
Gene ID	3861
Gene Name	KRT14
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Anti-KRT14 Antibody (Center) at 1:2000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	Formalin-fixed and paraffin-embedded human breast carcinoma with KRT14 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 2	IHC-P
Dilution image 2	1:50~100
Legend image 3	KRT14 Antibody (Center) (Cat.#AP6713c) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3	FC
Dilution image 3	1:10~50
Other Accession	Q6IFU7; Q6IFX2; Q6IFU8; Q9QWL7; Q04695; A1L595; Q6IFV1; Q61781
Predicted	Rat, B
Isotype	Rabbit Ig
Calculated MW	51561
Antigen Region	276-303
Antigen Source	HUMAN
Target/Specificity	This KRT14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-303 amino acids from the Central region of human KRT14.
NCBI Accession	NP_000517.2

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Menge: 400 ul

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