Hersteller |
Abcepta
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
other |
Isotype |
Ig |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applikationen |
WB, FC, IHC-P |
Menge |
400 ul |
Host |
Rabbit |
ArtNr |
ABC-AP6830b |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Primary Accession |
P12694 |
Antigen Type |
Synthetic Peptide |
Application |
FC, IHC-P, WB |
Bio Background |
The branched-chain alpha-keto acid (BCAA) dehydrogenase(BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). BCKDHA is the alpha subunit of the decarboxylase (E1) component. |
Bio References |
Flaschker, N., et.al., J. Inherit. Metab. Dis. 30 (6), 903-909 (2007) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB20924 |
Gene ID |
593 |
Gene Name |
BCKDHA |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
Western blot analysis of BCKDHA Antibody (C-term) (Cat. #AP6830b) in Jurkat cell line lysates (35ug/lane). BCKDHA (arrow) was detected using the purified Pab. |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Legend image 2 |
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with BCKDHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Type image 2 |
IHC-P |
Dilution image 2 |
1:50~100 |
Legend image 3 |
BCKDHA Antibody (C-term) (Cat. #AP6830b) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Type image 3 |
FC |
Dilution image 3 |
1:10~50 |
Isotype |
Rabbit Ig |
Calculated MW |
50471 |
Antigen Region |
362-390 |
Antigen Source |
HUMAN |
Target/Specificity |
This BCKDHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 362-390 amino acids from the C-terminal region of human BCKDHA. |
NCBI Accession |
NP_000700.1 |
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