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PAX8 Antibody (Center)

PAX8 Antibody (Center)

ArtNr	ABC-AP6874C
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PAX8, Paired box protein Pax-8
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Primary Accession	Q06710
Antigen Type	Synthetic Peptide
Application	FC, IHC-P, WB
Bio Background	PAX8 is a member of the paired box (PAX) family of transcription factors. Members of this family contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes.
Bio References	Ramos, H.E., et.al., Thyroid 19 (1), 61-68 (2009)
Clonality	Polyclonal
Clone/Animal Names	RB20218
Gene ID	7849
Gene Name	PAX8
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of PAX8 Antibody (Center) (Cat. #AP6874c) in HL-60 cell line lysates (35ug/lane). PAX8 (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	All lanes : Anti-PAX8 Antibody (Center) at 1:1000 dilution Lane 1: SK-OV-3 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded mouse kidney reacted with PAX8 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Legend image 4	Flow cytometric analysis of 293 cells using PAX8 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	Q00288
Predicted	M
Isotype	Rabbit Ig
Calculated MW	48218
Antigen Region	285-313
Antigen Source	HUMAN
Target/Specificity	This PAX8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-313 amino acids from the Central region of human PAX8.
NCBI Accession	NP_003457.1; NP_039246.1; NP_039247.1; NP_054698.1

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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ABC-AP6874C-datasheet.pdf

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Menge: 400 ul

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