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GLUL Antibody (N-term)

GLUL Antibody (N-term)

ArtNr	ABC-AP6892a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	GLUL, Glutamine synthetase, GS, GLNS, Glutamate--ammonia ligase, Glutamate decarboxylase
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Primary Accession	P15104
Antigen Type	Synthetic Peptide
Application	WB, IHC-P
Bio Background	GLUL belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling.
Bio References	Di Tommaso, L., et.al., J. Hepatol. 50 (4), 746-754 (2009)
Clonality	Polyclonal
Clone/Animal Names	RB21070
Gene ID	2752
Gene Name	GLUL {ECO:0000303\|PubMed:30158707, ECO:0000312\|HGNC:HGNC:4341}
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Western blot analysis of lysates from Hela, Raji, Jurkat, HT-29 cell line (from left to right), using GLUL Antibody (N-term)(Cat. #AP6892a). AP6892a was diluted at 1:2000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	Western blot analysis of lysates from Hela, HT-29, Jurkat cell line, mouse brain, mouse cerebellum, rat cerebellum tissue lysate(from left to right), using GLUL Antibody (N-term)(Cat. #AP6892a). AP6892a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilution Lane 1: mouse cerebellum lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3	WB
Dilution image 3	1:2000
Legend image 4	All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilution Lane 1: HT-29 whole cell lysates Lane 2: mouse cerebellum lysates Lane 3: Raji whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 4	WB
Dilution image 4	1:2000
Legend image 5	All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: mouse cerebellum lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 5	WB
Dilution image 5	1:2000
Legend image 6	Anti-GLUL Antibody (N-term)at 1:1000 dilution + Raji whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 6	WB
Dilution image 6	1:1000
Legend image 7	All lanes : Anti-GLUL Antibody (N-term) at 1:2000 dilution Lane 1: mouse brain lysates Lane 2: Hela whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 7	WB
Dilution image 7	1:2000
Other Accession	P46410; P15105; Q4R7U3; P16580; P15103
Predicted	B, C, Pr, Pig
Isotype	Rabbit Ig
Calculated MW	42064
Antigen Region	70-96
Antigen Source	HUMAN
Target/Specificity	This GLUL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-96 amino acids from the N-terminal region of human GLUL.
NCBI Accession	NP_001028216.1; NP_001028228.1; NP_002056.2

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ABC-AP6892a-datasheet.pdf

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Menge: 400 ul

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