NDUFS4 Antibody (C-term)

Synthetic Peptide

NDUFS4 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase(Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane.

Polyclonal

4724

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-NDUFS4 Antibody (C-term) at 1:2000 dilution + Mouse kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 20 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:2000

IHC-P-Leica

Flow cytometric analysis of ATDC5 cells using NDUFS4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

1:10~50

20108

HUMAN

NP_002486.1