TH Antibody (C-term)

ArtNr	ABC-AP6945b
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	TH, Tyrosine 3-hydroxylase, Tyrosine 3-monooxygenase, TYH
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Primary Accession	P07101
Antigen Type	Synthetic Peptide
Application	WB, FC, IHC-P
Bio Background	TH is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons.
Bio References	Kuhn, D.M., et.al., J. Biol. Chem. 277 (16), 14336-14342 (2002)
Clonality	Polyclonal
Clone/Animal Names	RB21096
Gene ID	7054
Gene Name	TH
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of lysates from SW480, U-87 MG cell line (from left to right), using TH Antibody (C-term)(Cat. #AP6945b). AP6945b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	TH Antibody (C-term) (Cat. #AP6945b) western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the TH antibody detected the TH protein (arrow).
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human brain tissue with TH Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	Flow cytometric analysis of K562 cells using TH Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Isotype	Rabbit Ig
Calculated MW	58600
Antigen Region	486-514
Antigen Source	HUMAN
Target/Specificity	This TH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 486-514 amino acids from the C-terminal region of human TH.
NCBI Accession	NP_000351.2; NP_954986.2; NP_954987.2

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