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AKT2 Antibody (Center)

AKT2 Antibody (Center)

ArtNr	ABC-AP7029c
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	AKT2, RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, RAC-PK-beta, PKB beta
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Primary Accession	P31751
Antigen Type	Synthetic Peptide
Application	IHC-P, FC, IF, WB
Bio Background	AKT2 is a putative oncogenic protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Bio References	Vojtek, A.B., et al., Mol. Cell. Biol. 23(13):4417-4427 (2003). Yuan, Z.Q., et al., J. Biol. Chem. 278(26):23432-23440 (2003). Jones, D.T., et al., Blood 101(8):3174-3180 (2003). Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003). Miyakawa, M., et al., Endocr. J. 50(1):77-83 (2003).
Clonality	Polyclonal
Clone/Animal Names	RB3026
Gene ID	208
Gene Name	AKT2
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of AKT2 Antibody (Center)(Cat#AP7029c) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (Center)(Cat.#AP7029c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	AKT2 Antibody (N108) (Cat. #AP7029c) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AKT2 antibody detected the AKT2 protein (arrow).
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	AKT2 Antibody (Center) (AP7029c)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung adenocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of AKT2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 4	IHC-P
Dilution image 4	1:10~50
Legend image 5	AKT2 Antibody (Center) (Cat. #AP7029c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 5	FC
Dilution image 5	1:10~50
Isotype	Rabbit Ig
Calculated MW	55769
Antigen Region	93-123
Antigen Source	HUMAN
Target/Specificity	This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the Central region of human AKT2.
NCBI Accession	NP_001617.1

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