AKT1 Antibody (N-term)

Synthetic Peptide

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

RB11644

AKT1

H

IF

Western blot analysis of AKT1 Antibody (N-term) polyclonal antibody(Cat.#AP7141a)(arrow). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT1 gene (Lane 2) (Origene Technologies).

1:1000

WB

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with AKT1 antibody (N-term)(Cat.#AP7141a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

1:50~100

FC

P31750; Q01314

Rabbit Ig

115-144

This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the N-terminal region of human AKT1.