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Cyclin A (CCNA2) Antibody (N-term)

Cyclin A (CCNA2) Antibody (N-term)

ArtNr	ABC-AP7292a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	CCNA2, CCN1, CCNA, Cyclin-A2, Cyclin-A
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Primary Accession	P20248
Antigen Type	Synthetic Peptide
Application	FC, IHC-P, WB
Bio Background	CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
Bio References	Henglein B., Proc. Natl. Acad. Sci. U.S.A. 91:5490-5494(1994) Wang J., Nature 343:555-557(1990) Tsang, W.Y., J. Cell Biol. 178 (4), 621-633 (2007)
Clonality	Polyclonal
Clone/Animal Names	RB14617
Gene ID	890
Gene Name	CCNA2 (HGNC:1578)
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	All lanes : Anti-CCNA2 Antibody (N-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: MGC803 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	Formalin-fixed and paraffin-embedded human hepatocarcinoma with Cyclin A (CCNA2) Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 2	IHC-P
Dilution image 2	1:50~100
Legend image 3	Flow cytometric analysis of HepG2 cells using Cyclin A (CCNA2) Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3	FC
Dilution image 3	1:10~50
Isotype	Rabbit Ig
Calculated MW	48551
Antigen Region	51-84
Antigen Source	HUMAN
Target/Specificity	This Cyclin A (CCNA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-84 amino acids from the N-terminal region of human Cyclin A (CCNA2).
NCBI Accession	NP_001228.1

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