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ACO2 Antibody (Center)

ACO2 Antibody (Center)

ArtNr	ABC-AP7561C
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	ACO2, mitochondrial, Aconitate hydratase, Citrate hydro-lyase, Aconitase
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Primary Accession	Q99798
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.
Bio References	Yu, Z., Prostate 66 (10), 1061-1069 (2006) Tsui, K.H., Asian J. Androl. 8 (3), 307-315 (2006) Ahmed, M., J. Proteome Res. 4 (3), 931-940 (2005)
Clonality	Polyclonal
Clone/Animal Names	RB14646
Gene ID	50
Gene Name	ACO2
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	All lanes : Anti-ACO2 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Western blot analysis of ACO2 (arrow) using rabbit polyclonal ACO2 Antibody (Center) (Cat.#AP7561c).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACO2 gene (Lane 2) (Origene Technologies).
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ACO2 antibody (Center) (Cat.#AP7561c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Isotype	Rabbit Ig
Calculated MW	85425
Antigen Region	294-325
Antigen Source	HUMAN
Target/Specificity	This ACO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-325 amino acids from the Central region of human ACO2.
NCBI Accession	NP_001089.1

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ABC-AP7561C-datasheet.pdf

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Menge: 400 ul

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