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RAC1 Antibody (S71)

ArtNr ABC-AP7578a
Hersteller Abcepta
Menge 400 ul
Kategorie
Typ Antibody Primary
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen WB
Specific against other
Host Rabbit
Isotype Ig
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Similar products RAC1, p21-Rac1, Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, TC25
Lieferbar
Primary Accession
P63000
Antigen Type
Synthetic Peptide
Application
WB
Bio Background
RAC1 is a GTPase belonging to the RAS superfamily of small GTP-binding proteins. It is a pleiotropic regulator of many cellular processes, including the cell cycle, cell-cell adhesion, motility (through the actin network), and of epithelial differentiation (proposed to be necessary for maintaining epidermal stem cells).
Bio References
Wang, T., Neurosci. Lett. 437 (2), 71-75 (2008)
Gorshkova, I., J. Biol. Chem. 283 (17), 11794-11806 (2008)
Simeone-Penney, M.C., Am. J. Physiol. Lung Cell Mol. Physiol. 294 (4), L698-L704 (2008)
Clonality
Polyclonal
Clone/Animal Names
RB11123
Gene ID
5879
Gene Name
RAC1 (HGNC:9801)
Subtitle
Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity
H, Rat
Legend image 1
Western blot analysis of RAC1 (arrow) using rabbit polyclonal RAC1 Antibody (S71) (Cat.#AP7578a).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RAC1 gene (Lane 2) (Origene Technologies).
Type image 1
WB
Dilution image 1
1:1000
Legend image 2
All lanes : Anti-RAC1 Antibody (S71) at 1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: MCF-7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 21 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2
WB
Dilution image 2
1:2000
Legend image 3
All lanes : Anti-RAC1 Antibody (S71) at 1:2000 dilution
Lane 1: MCF-7 whole cell lysate
Lane 2: rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 21 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 3
WB
Dilution image 3
1:2000
Other Accession
P60764; P60763; Q05144; P15153; P48554; Q9TU25; Q6RUV5; P63001; P40792; Q03206; P62998; Q8CFN2; Q007T2; P60766; Q4R4R6; P60953; P40793; Q90694; Q05062; Q2KJ93
Predicted
B, E, C, D, Pr, M, Pig
Isotype
Rabbit Ig
Calculated MW
21450
Antigen Region
49-78
Antigen Source
HUMAN
Target/Specificity
This RAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from human RAC1.
NCBI Accession
NP_008839.2; NP_061485.1

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 400 ul
Lieferbar: In stock
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