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LYN Antibody (N-term)

LYN Antibody (N-term)

ArtNr	ABC-AP7713A
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	LYN, Tyrosine-protein kinase Lyn, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn, JTK8, Lck/Yes-related novel protein tyrosine kinase
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Primary Accession	P07948
Antigen Type	Synthetic Peptide
Application	IHC-P, FC, WB
Bio Background	Lyn is a protein tyrosine kinase which is important in cellular signaling. It contains an SH2 and SH3 domain and has been found to be cleaved after activation of caspases in apoptosis.
Bio References	Baran, C.P., et al., J. Biol. Chem. 278(40):38628-38636 (2003). Donato, N.J., et al., Blood 101(2):690-698 (2003). Santini, V., et al., Haematologica 87(12):1242-1247 (2002). Harashima, A., et al., Blood 100(13):4440-4445 (2002). Ren, X., et al., Cancer Biol. Ther. 1(6):703-708 (2002).
Clonality	Polyclonal
Clone/Animal Names	RB03080
Gene ID	4067
Gene Name	LYN
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Anti-LYN Antibody (E46) at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Western blot analysis of anti-LYN Antibody (N-term) (Cat.#AP7713a) in HL60 cell line lysates (35ug/lane). LYN (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	LYN Antibody (N-term) (Cat. #AP7713a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Isotype	Rabbit Ig
Calculated MW	58574
Antigen Region	32-62
Antigen Source	HUMAN
Target/Specificity	This LYN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-62 amino acids from the N-terminal region of human LYN.
NCBI Accession	NP_001104567.1; NP_002341.1

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Menge: 400 ul

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