ZAP70 Antibody (Center)

Synthetic Peptide

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Polyclonal

7535

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-ZAP70 Antibody (A267) at 1:1000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: MOLT-4 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 70 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:1000

IHC-P

Rabbit Ig

252-282

This ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 252-282 amino acids from the Central region of human ZAP70.