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PIM1 Antibody (C-term)

PIM1 Antibody (C-term)

ArtNr	ABC-AP7932a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PIM1, Serine/threonine-protein kinase pim-1
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Primary Accession	P11309
Antigen Type	Synthetic Peptide
Application	IHC-P, WB
Bio Background	PIM1, which belongs to the Serine/Threonine protein kinase family, is thought to play a role in signal transduction in blood cells. The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer. It may affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. PIM1 is expressed primarily in cells of the hematopoietic and germ line lineages.
Bio References	Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Pasqualucci, L., et al., Nature 412(6844):341-346 (2001). Koike, N., et al., FEBS Lett. 467(1):17-21 (2000). Reeves, R., et al., Gene 90(2):303-307 (1990). Telerman, A., et al., Mol. Cell. Biol. 8(4):1498-1503 (1988).
Clonality	Polyclonal
Clone/Animal Names	RB1285, RB1286
Gene ID	5292
Gene Name	PIM1
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Anti-PIM1 Antibody (E298) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 2	IHC-P
Dilution image 2	1:50~100
Other Accession	Q9N0P9
Predicted	B
Isotype	Rabbit Ig
Calculated MW	35686
Antigen Region	374-404
Antigen Source	HUMAN
Target/Specificity	This PIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-404 amino acids from the C-terminal region of human PIM1.
NCBI Accession	NP_001230115.1; NP_002639.1

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