MAP2K2 Antibody (T394)

Synthetic Peptide

MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.

Polyclonal

5605

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Fluorescent image of U251 cell stained with MAP2K2 Antibody (T394)(Cat#AP7961d/SA080220AJ).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MAP2K2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). MAP2K2 immunoreactivity is localized to Cytoplasm significantly.

1:10~50

WB

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (T394) (Cat.#AP7961d), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

1:10~50

44424

HUMAN

NP_109587.1