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PCK1 Antibody (N-term)

PCK1 Antibody (N-term)

ArtNr	ABC-AP8093a
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PCK1, PEPCK1, Phosphoenolpyruvate carboxykinase, cytosolic [GTP], PEPCK-C
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Primary Accession	P35558
Antigen Type	Synthetic Peptide
Application	WB, IHC-P
Bio Background	This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.
Bio References	Berger, K. et al. PLoS ONE. 4(3): e4671 (2009). Dunten, P., et al., J. Mol. Biol. 316(2):257-264 (2002). Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Deloukas, P., et al., Nature 414(6866):865-871 (2001). O'Brien, R.M., et al., Biochim. Biophys. Acta 1264(3):284-288 (1995). Ting, C.N., et al., Genomics 16(3):698-706 (1993).
Clonality	Polyclonal
Clone/Animal Names	RB3644
Gene ID	5105
Gene Name	PCK1 {ECO:0000303\|PubMed:8490617, ECO:0000312\|HGNC:HGNC:8724}
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	The anti-PCK1 Pab (Cat. #AP8093a) is used in Western blot to detect PCK1 in mouse liver tissue lysate.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	PCK1 Antibody (N-term) (Cat. #AP8093a) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PCK1 antibody detected the PCK1 protein (arrow).
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Type image 3	IHC-P
Dilution image 3	1:50~100
Isotype	Rabbit Ig
Calculated MW	69195
Antigen Region	40-70
Antigen Source	HUMAN
Target/Specificity	This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-70 amino acids from the N-terminal region of human PCK1.
NCBI Accession	NP_002582.3

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Menge: 400 ul

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