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Fructose 6 Phosphate Kinase (PFKM) Antibody (C-term)

Fructose 6 Phosphate Kinase (PFKM) Antibody (C-term)

ArtNr	ABC-AP8137b
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PFKM, PFKX, Phosphofructo-1-kinase isozyme A, PFK-A, ATP-dependent 6-phosphofructokinase, PFK-M, ATP-PFK {ECO:0000255\|HAMAP-Rule:MF_03184}, 27111 {ECO:0000255\|HAMAP-Rule:MF_03184}, Phosphohexokinase {ECO:0000255\|HAMAP-Rule:MF_03184}, muscle type {ECO:0000255\|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type A
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Primary Accession	P08237
Antigen Type	Synthetic Peptide
Application	IF, IHC-P, WB
Bio Background	Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1, 6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Only M type PFK isozyme is expressed in mature muscle, while erythrocytes contain both L and M subunits. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7), also known as Tarui disease.
Bio References	Howard, T.D., et al., Genomics 34(1):122-127 (1996). Vasconcelos, O., et al., Proc. Natl. Acad. Sci. U.S.A. 92(22):10322-10326 (1995). Raben, N., et al., J. Biol. Chem. 268(7):4963-4967 (1993). Yamasaki, T., et al., Gene 104(2):277-282 (1991). Sharma, P.M., et al., J. Biol. Chem. 265(16):9006-9010 (1990).
Clonality	Polyclonal
Clone/Animal Names	RB3908
Gene ID	5213
Gene Name	PFKM
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of Fructose 6 Phosphate Kinase (PFKM) Antibody(C-term)(Cat#AP8137b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of PFKM polyclonal antibody (Cat. #AP8137b) in Hela cell line lysate. PFKM(arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody (C-term)(Cat.#AP8137b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4	IHC-P
Dilution image 4	1:50~100
Other Accession	Q60HD9
Predicted	Pr
Isotype	Rabbit Ig
Calculated MW	85183
Antigen Region	746-776
Antigen Source	HUMAN
Target/Specificity	This Fructose 6 Phosphate Kinase (PFKM) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 746-776 amino acids from the C-terminal region of human Fructose 6 Phosphate Kinase (PFKM).
NCBI Accession	NP_000280.1; NP_001160158.1; NP_001160159.1; NP_001160160.1

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Menge: 400 ul

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