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IL1B Antibody (Center)

IL1B Antibody (Center)

ArtNr	ABC-AP8531C
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	IL-1 beta, IL1B, IL1F2, Interleukin-1 beta, Catabolin
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Primary Accession	P01584
Antigen Type	Synthetic Peptide
Application	IHC-P, WB, FC
Bio Background	IL1B is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system ( CNS) is found to contribute to inflammatory pain hypersensitivity.
Bio References	Yu, J., et.al., Am. J. Gastroenterol. (2009) Ito, A., et.al., J. Biol. Chem. 271 (25), 14657-14660 (1996)
Clonality	Polyclonal
Clone/Animal Names	RB21917
Gene ID	3553
Gene Name	IL1B (HGNC:5992)
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	All lanes : Anti-IL1B Antibody (Center) at 1:2000 dilution Lane 1: human spleen lysate Lane 2: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:2000
Legend image 2	Western blot analysis of IL1B Antibody (Center) (Cat. #AP8531c) in NCI-H460 cell line lysates (35ug/lane). IL1B (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human lung carcinoma with IL1B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:10~50
Legend image 4	Overlay histogram showing MCF-7 cells stained with AP8531c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP8531c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 4	FC
Dilution image 4	1:25
Other Accession	P14628; P79182
Predicted	Pr, Rb
Isotype	Rabbit IgG
Calculated MW	30748
Antigen Region	148-174
Antigen Source	HUMAN
Target/Specificity	This IL1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 148-174 amino acids of human IL1B.
NCBI Accession	NP_000567.1

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