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SOCS1 Antibody (N-term)

SOCS1 Antibody (N-term)

ArtNr	ABC-AP8790A
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	SOCS-1, SOCS1, JAK-binding protein, JAB, SSI-1, TIP-3, Tec-interacting protein 3, SSI1, TIP3, Suppressor of cytokine signaling 1, STAT-induced STAT inhibitor 1
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Primary Accession	O15524
Antigen Type	Synthetic Peptide
Application	WB, FC, IF, IHC-P
Bio Background	SOCS1 is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling(SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling.
Bio References	Starr, R., et.al., Nature 387 (6636), 917-921 (1997) Minamoto, S., et.al., Biochem. Biophys. Res. Commun. 237 (1), 79-83 (1997)
Clonality	Polyclonal
Clone/Animal Names	RB17347
Gene ID	8651
Gene Name	SOCS1
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	M, Rat
Legend image 1	Confocal immunofluorescent analysis of SOCS1 Antibody (N-term) (Cat. #AP8790a) with 293 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	All lanes : Anti-SOCS1 Antibody (N-term) at 1:2000 dilution Lane 1: mouse thymus lysates Lane 2: rat spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:2000
Legend image 3	Western blot analysis of SOCS1 Antibody (N-term) (Cat. #AP8790a) in mouse kidney tissue lysates (35ug/lane). SOCS1 (arrow) was detected using the purified Pab.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Formalin-fixed and paraffin-embedded human colon carcinoma reacted with SOCS1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4	IHC-P
Dilution image 4	1:10~50
Legend image 5	SOCS1 Antibody (N-term) (Cat. #AP8790a) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 5	FC
Dilution image 5	1:10~50
Isotype	Rabbit Ig
Calculated MW	23551
Antigen Region	35-66
Antigen Source	HUMAN
Target/Specificity	This SOCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-66 amino acids from the N-terminal region of human SOCS1.
NCBI Accession	NP_003736.1

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Menge: 400 ul

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