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GLS Antibody (C-term)

GLS Antibody (C-term)

ArtNr	ABC-AP8809b
Hersteller	Abcepta
Menge	200 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	GLS, mitochondrial, GLS1, KIAA0838, K-glutaminase, L-glutamine amidohydrolase, Glutaminase kidney isoform
Lieferbar
Primary Accession	O94925
Antigen Type	Synthetic Peptide
Application	WB, IHC-P-Leica, IF, FC
Bio Background	Sahai (1983) demonstrated phosphate-activated glutaminase (EC 3.5.1.2) in human platelets. It is the major enzyme yielding glutamate from glutamine. Significance of the enzyme derives from its possible implication in behavior disturbances in which glutamate acts as a neurotransmitter(Prusiner, 1981). High heritability of platelet glutaminase was indicated by studies of Sahai and Vogel (1983) [PubMed 6682827] who found an intraclass correlation coefficient of 0.96 for monozygotic twins and 0.53 for dizygotic twins.
Bio References	Swierczynski, J., et.al., Biochim. Biophys. Acta 1157 (1), 55-62 (1993)
Clonality	Polyclonal
Clone/Animal Names	RB22875
Gene ID	2744
Gene Name	GLS
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M, Rat
Legend image 1	All lanes : Anti-GLS Antibody (C-term) at 1:1000 dilution Lane 1: 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Observed band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	All lanes : Anti-GLS Antibody (C-term) at 1:1000 dilution Lane 1: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Observed band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling GLS with AP8809b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
Type image 3	IF
Dilution image 3	1:25
Legend image 4	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling GLS with AP8809b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
Type image 4	IF
Dilution image 4	1:25
Legend image 5	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-251 MG cells labeling GLS with AP8809B at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Cytoplasm staining on U-251 MG cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).
Type image 5	IF
Dilution image 5	1:25
Legend image 6	Western blot analysis of lysates from human brain, mouse brain ad rat kidney tissue lysate (from left to right), using GLS Antibody (C-term)(Cat. #AP8809b). AP8809b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Type image 6	WB
Dilution image 6	1:1000
Legend image 7	All lanes : Anti-GLS Antibody (C-term) at 1:2000 dilution Lane 1: human brain lysate Lane 2: human kidney lysate Lane 3: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 7	WB
Dilution image 7	1:2000
Other Accession	P13264; D3Z7P3
Predicted	Rat
Isotype	Rabbit Ig
Calculated MW	73461
Antigen Region	516-545
Antigen Source	HUMAN
Target/Specificity	This GLS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human GLS.
NCBI Accession	NP_001243239.1; NP_055720.3

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ABC-AP8809b-datasheet.pdf

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Menge: 200 ul

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