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COTL1 Antibody (C-term)

COTL1 Antibody (C-term)

ArtNr	ABC-AP8819b
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	COTL1, CLP, Coactosin-like protein
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Primary Accession	Q14019
Antigen Type	Synthetic Peptide
Application	WB, FC, IHC-P
Bio Background	COTL1 is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis.
Bio References	Provost, P., et.al., Proc. Natl. Acad. Sci. U.S.A. 96 (5), 1881-1885 (1999) Provost, P., et.al., J. Biol. Chem. 276 (19), 16520-16527 (2001)
Clonality	Polyclonal
Clone/Animal Names	RB23505
Gene ID	23406
Gene Name	COTL1
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Western blot analysis of COTL1 Antibody (C-term) (Cat. #AP8819b) in Hela cell line lysates (35ug/lane). COTL1 (arrow) was detected using the purified Pab.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Western blot analysis of COTL1 (arrow) using rabbit polyclonal COTL1 Antibody (C-term) (Cat. #AP8819b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the COTL1 gene.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human spleen tissue reacted with COTL1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	COTL1 Antibody (C-term) (Cat. #AP8819b) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	Q2HJ57; NP_066972
Predicted	B
Isotype	Rabbit Ig
Calculated MW	15945
Antigen Region	115-142
Antigen Source	HUMAN
Target/Specificity	This COTL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-142 amino acids from the C-terminal region of human COTL1.
NCBI Accession	NP_066972.1

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ABC-AP8819b-datasheet.pdf

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