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MFN2 Antibody (Center)

MFN2 Antibody (Center)

ArtNr	ABC-AP8840c
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	Mitofusin-2, MFN2, Transmembrane GTPase MFN2, CPRP1, KIAA0214, 365-
Lieferbar
Primary Accession	O95140
Antigen Type	Synthetic Peptide
Application	FC, WB, IHC-P
Bio Background	MFN2 is a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system.
Bio References	Calvo, J., et.al., Arch. Neurol. 66 (12), 1511-1516 (2009)
Clonality	Polyclonal
Clone/Animal Names	RB21136
Gene ID	9927
Gene Name	MFN2
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Anti-MFN2 Antibody (Center) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	AP8840C staining MFN2 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 2	IHC-P
Dilution image 2	1:25
Legend image 3	MFN2 Antibody (Center)(Cat. #AP8840c) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 3	FC
Dilution image 3	1:10~50
Other Accession	Q8R500; Q80U63
Predicted	Rat
Isotype	Rabbit Ig
Calculated MW	86402
Antigen Region	447-476
Antigen Source	HUMAN
Target/Specificity	This MFN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 447-476 amino acids from the Central region of human MFN2.
NCBI Accession	NP_001121132.1; NP_055689.1

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