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PIN1 Antibody (Center)

PIN1 Antibody (Center)

ArtNr	ABC-AP8959C
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	PIN1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1, Rotamase Pin1, PPIase Pin1
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Primary Accession	Q13526
Antigen Type	Synthetic Peptide
Application	IF, WB, FC, IHC-P
Bio Background	PIN1 is an essential nuclear peptidylprolyl cis-trans isomerase (PPIase; EC 5.2.1.8) involved in regulation of mitosis.
Bio References	Lu, K.P., et.al., Nature 380 (6574), 544-547 (1996) Campbell, H.D., et.al., Genomics 44 (2), 157-162 (1997)
Clonality	Polyclonal
Clone/Animal Names	RB22881
Gene ID	5300
Gene Name	PIN1
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of PIN1 Antibody (Center) (Cat. #AP8959c) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of PIN1 Antibody (Center) (Cat. #AP8959c) in HL-60 cell line lysates (35ug/lane). PIN1 (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of PIN1 (arrow) using rabbit polyclonal PIN1 Antibody (Center) (Cat. #AP8959c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIN1 gene.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PIN1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4	IHC-P
Dilution image 4	1:50~100
Legend image 5	PIN1 Antibody (Center) (Cat.#AP8959c) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 5	FC
Dilution image 5	1:10~50
Other Accession	Q4R383
Predicted	Pr
Isotype	Rabbit Ig
Calculated MW	18243
Antigen Region	30-56
Antigen Source	HUMAN
Target/Specificity	This PIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-56 amino acids from the Central region of human PIN1.
NCBI Accession	NP_006212.1

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Menge: 400 ul

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