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NKX3-1 Antibody (Center)

NKX3-1 Antibody (Center)

ArtNr	ABC-AP8996C
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	NKX3-1, NKX3A, Homeobox protein NK-3 homolog A, NKX31, Homeobox protein Nkx-31
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Primary Accession	Q99801
Antigen Type	Synthetic Peptide
Application	FC, IHC-P, WB
Bio Background	NKX3-1 is a transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. It play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. It Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Bio References	Voeller, H.J., et.al., Cancer Res. 57 (20), 4455-4459 (1997)
Clonality	Polyclonal
Clone/Animal Names	RB23610
Gene ID	4824
Gene Name	NKX3-1
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Anti-NKX3-1 Antibody (Center) at 1:8000 dilution + LNCaP whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:8000
Legend image 2	Western blot analysis of NKX3-1 Antibody (Center) (Cat. #AP8996c) in MCF-7 cell line lysates (35ug/lane). NKX3-1 (arrow) was detected using the purified Pab.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with NKX3-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	NKX3-1 Antibody (Center) (Cat. #AP8996c) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Other Accession	P97436
Predicted	M
Isotype	Rabbit Ig
Calculated MW	26350
Antigen Region	3-1
Antigen Source	HUMAN
Target/Specificity	This NKX3-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-145 amino acids from the Central region of human NKX3-1.
NCBI Accession	NP_001243268.1; NP_006158.2

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