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DEK Antibody (C-term)

DEK Antibody (C-term)

ArtNr	ABC-AP9186b
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	DEK, Protein DEK
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Primary Accession	P35659
Antigen Type	Synthetic Peptide
Application	WB, IF, FC, IHC-P
Bio Background	DEK is a protein with one SAP domain. This protein binds to cruciform and superhelical DNA and induces positive supercoils into closed circular DNA, and is also involved in splice site selection during mRNA processing. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of antibodies against this protein are all associated with various diseases.
Bio References	Sawatsubashi, S., et.al., Genes Dev. 24 (2), 159-170 (2010) Khodadoust, M.S., et.al., Cancer Res. 69 (16), 6405-6413 (2009)
Clonality	Polyclonal
Clone/Animal Names	RB23679
Gene ID	7913
Gene Name	DEK
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of DEK Antibody (C-term) (Cat#AP9186b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	Western blot analysis of lysates from U-251 MG, PC-3 cell line (from left to right), using DEK Antibody (C-term)(Cat. #AP9186b). AP9186b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	DEK Antibody (C-term) (Cat. #AP9186b) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Type image 3	IHC-P
Dilution image 3	1:50~100
Legend image 4	DEK Antibody (C-term) (Cat. #AP9186b) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Type image 4	FC
Dilution image 4	1:10~50
Isotype	Rabbit Ig
Calculated MW	42674
Antigen Region	336-364
Antigen Source	HUMAN
Target/Specificity	This DEK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the C-terminal region of human DEK.
NCBI Accession	NP_001128181.1; NP_003463.1

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ABC-AP9186b-datasheet.pdf

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Menge: 400 ul

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