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TOP2A Antibody (C-term)

TOP2A Antibody (C-term)

ArtNr	ABC-AP9248B
Hersteller	Abcepta
Menge	400 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, IF, IHC-P
Specific against	other
Host	Rabbit
Isotype	Ig
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	TOP2A, TOP2, DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme
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Primary Accession	P11388
Antigen Type	Synthetic Peptide
Application	IF, IHC-P, WB
Bio Background	TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA.
Bio References	Kawamura, R., et.al, J. Cell Biol. 188 (5), 653-663 (2010) Alchanati, I., et.al, PLoS ONE 4 (12), E8104 (2009)
Clonality	Polyclonal
Clone/Animal Names	RB24346
Gene ID	7153
Gene Name	TOP2A
Subtitle	Affinity Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Confocal immunofluorescent analysis of TOP2A Antibody (C-term) (Cat#AP9248b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	All lanes : Anti-TOP2A Antibody (C-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 174 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of TOP2A Antibody (C-term) (Cat. #AP9248b) in A375 cell line lysates (35ug/lane). TOP2A (arrow) was detected using the purified Pab.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Formalin-fixed and paraffin-embedded human skin with TOP2A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Type image 4	IHC-P
Dilution image 4	1:50~100
Other Accession	P41516; Q01320; P41515
Predicted	Ha, M, Rat
Isotype	Rabbit Ig
Calculated MW	174385
Antigen Region	1167-1196
Antigen Source	HUMAN
Target/Specificity	This TOP2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1167-1196 amino acids from the C-terminal region of human TOP2A.
NCBI Accession	NP_001058.2

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Menge: 400 ul

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