Acinus Antibody

Q9UKV3

Acinus Antibody: Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS'.

Polyclonal

ACIN1

Immunohistochemistry of TWEAK in mouse brain tissue with TWEAK antibody at 5 µg/mL.

AAD56724; 22985

151862

Acinus antibody was raised against a peptide corresponding to amino acids near the C-terminus of the cleaved active peptide p17, which are identical to those of mouse Acinus.

The immunogen is located within amino acids 1050 - 1100 of Acinus.

Acinus antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Acinus antibody can be used for detection of Acinus by Western blot at 1 μ g/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μ g/mL. For immunofluorescence start at 10 μ g/mL.

Acinus Antibody is supplied in PBS containing 0.02% sodium azide.