HSP70 Antibody

ArtNr	ABC-ASM10001-A594-200
Hersteller	Abcepta
Menge	Inquiry
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	ATTO 594
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Lieferbar
Primary Accession	P08107
Reactivity	H, M, Rat, Rb, Ha, Pr, Pig, C, B, E, Sh, Gp, Dog, D
Legend image 1	Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (ASM10001). Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (ASM10001) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
Type image 1	IHC
Dilution image 1	1:10000
Legend image 2	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (ASM10001). Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (ASM10001) at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse. Courtesy of: Dr. Ewa Malusecka, Maria Sklodowska-Curie Memorial Cancer Centre and Inst. Of Oncology, Poland.
Type image 2	ICC/IF
Dilution image 2	1:1000
Legend image 3	Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (ASM10001). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (ASM10001) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
Type image 3	IHC
Dilution image 3	1:10000
Legend image 4	Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (ASM10001). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (ASM10001) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Type image 4	WB
Dilution image 4	1:1000
Legend image 5	Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92 (ASM10001). Tissue: Heat Shocked CD3+ CD8+ T cells . Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody (ASM10001) at 1:1000. Courtesy of: Cheryl Cameron, Vaccine and Gene Therapy Instit. Florida.
Type image 5	FACS
Dilution image 5	1:1000
Other Accession	NP_005336.3
Isotype	IgG
Target/Specificity	Detects ~70kDa. Does not cross-react with HSC70 (HSP73).
Immunogen	Human HSP70
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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ABC-ASM10001-A594-200-datasheet.pdf

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