N-terminal Arginylation Antibody

Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 (ASM10171). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody (ASM10171) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.

1:1000

Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.

Protein G Purified