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4-Hydroxynonenal Antibody

4-Hydroxynonenal Antibody

ArtNr	ABC-ASM10339-APC-100
Hersteller	Abcepta
Menge	Inquiry
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	APC
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
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Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody, Clone 12F7 (ASM10339). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (ASM10339) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A, C, E, G) - Untreated. (B, D, F, H) - Cells cultured overnight with 50 µM H2O2. (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alex Fluor 633 F-Actin stain. (E, F) 4-Hydroxynonenal Antibody. (G, H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Type image 1	ICC/IF
Dilution image 1	1:50
Legend image 2	Western Blot analysis of 4-hydroxy-nonenal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-nonenal-BSA using Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody, Clone 12F7 (ASM10339). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: 4-hydroxyl nonenal-BSA (0.5 µg). Lane 4: 4-hydroxy nonenal-BSA (2.0 µg). Lane 5: 4-hydroxy-2-hexenal (0.5 µg). Lane 6: 4-hydroxy-2-hexenal (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-nonenal Monoclonal Antibody (ASM10339) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Type image 2	WB
Dilution image 2	1:1000
Isotype	IgG1
Target/Specificity	Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Immunogen	Synthetic 4-Hydroxynonenal modified Keyhole Limpet Hemocyanin (KLH).
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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ABC-ASM10339-APC-100-datasheet.pdf

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