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Alpha B Crystallin Antibody

Alpha B Crystallin Antibody

ArtNr	ABC-ASM10392-50
Hersteller	Abcepta
Menge	Inquiry
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Unconjugated
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Lieferbar
Primary Accession	P02511
Reactivity	H, M, Rat, C, B
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ASM10392). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ASM10392) at 1:120 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Type image 1	ICC/IF
Dilution image 1	1:120
Legend image 2	Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ASM10392). Lane 1: Molecular Weight Ladder (MW). Lane 2: A431 cell lysates. Lane 3: HCT116 cell lysates. Lane 4: HeLa cell lysates. Lane 5: HepG2 cell lysates. Lane 6: HEK293 cell lysates. Lane 7: HUVEC cell lysates. Lane 8: Jurkat cell lysates. Lane 9: MCF7 cell lysates. Lane 10: PC3 cell lysates. Lane 11: T98G cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ASM10392) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.
Type image 2	WB
Dilution image 2	1:5000
Legend image 3	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ASM10392). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ASM10392) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Alpha B Crystallin Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Type image 3	ICC/IF
Dilution image 3	1:120
Legend image 4	Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa Alpha B Crystallin protein using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ASM10392). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody (ASM10392) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.
Type image 4	WB
Dilution image 4	1:5000
Other Accession	NP_001876.1
Target/Specificity	Detects ~22kDa. Does not cross-react with αA-crystallin.
Immunogen	Synthetic peptide corresponding to human alpha B crystallin conjugated to KLH
Purification	Rabbit Antiserum
Reconstitution & Storage	-20ºC

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